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- Primary Antibodies ›
- CD133 (Prominin-1) Antibodies
Invitrogen
View all (27) CD133 (Prominin-1) antibodies
Cite CD133 (Prominin-1) Monoclonal Antibody (13A4), FITC, eBioscience™
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CD133 (Prominin-1) Antibody (11-1331-82) in Flow
Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD11b APC (Product # 17-0112-82) and 0.25 µg of Rat IgG1 kappa Isotype Control FITC (Product # 11-4301-82) (left) or 0.25 µg of Anti-Mouse CD133 (Prominin-1) FITC (right). Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
11-1331-82
Applications
Tested Dilution
Publications
Western Blot(WB)
-
View 4 publications4 publications
Immunohistochemistry (IHC)
-
View 14 publications14 publications
Immunohistochemistry (Frozen)(IHC (F))
-
View 1 publication1 publication
Immunocytochemistry(ICC/IF)
-
View 7 publications7 publications
Flow Cytometry(Flow)
0.5 µg/test
View 27 publications27 publications
Product Specifications
Species Reactivity
Dog,Mouse
Published species
Dog,Human,Mouse
Host/Isotype
Rat/ IgG1, kappa
Recommended Isotype Control
Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
13A4
Conjugate
FITC
- Alexa Fluor 488
- APC
- Biotin
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Unconjugated
Excitation/Emission Max
498/517 nmView spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
AB_465198
Product Specific Information
Description: The 13A4 monoclonal antibody recognizes mouse Prominin-1 (sometimes also referred to as CD133 and, in the case of the human orthologue, as AC133), a 115-120 kDa pentaspan transmembrane (5-TM) domain glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic stem and progenitor cells, neural and endothelial stem cells, retina and retinoblastoma, as well as developing epithelium. To date, the function and ligand of Prominin-1 are unknown. The 13A4 antibody does not cross react with rat, human, chicken, or Drosophila antigen but has been reported to work in canine/dog.
Applications Reported: The 13A4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The 13A4 antibody has been tested flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases:Antigen AC133 hom*olog; antigen CD133; CD133; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; Mac3; prominin 1.s1; Prominin-1; prominin-1.s2; prominin-like 1; Prominin-like protein 1; Prominin1
UniProt ID:(Mouse) O54990
Entrez Gene ID:(Dog) 488816, (Mouse) 19126
Function(s)
retina layer formationphotoreceptor cell maintenanceretina morphogenesis in camera-type eyecamera-type eye photoreceptor cell differentiationglomerular visceral epithelial cell differentiationglomerular parietal epithelial cell differentiationpositive regulation of nephron tubule epithelial cell differentiation
Process(es)
actinin bindingcadherin binding
It has to be done as per old AB suggested Products section.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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